Biosynthesis and characterization of chitosan nanoparticles, determination of its antifungal and antiaflatoxigenic against Aspergillus flavus isolates

Aflatoxin is a mycotoxin generated mostly by the fungus Aspergillus flavus, which may be found in food and feed. It carcinogenic poison for both humans animals. The goal of this investigation was to see if toxicogenic A. flavus strains might on nuts Kurdistan Region. A total Fifty nut samples. colony color texture were used identify macromorphological qualities, whereas spore color, size, structure, conidiophore vesicle shape assess micromorphological properties. Direct imaging UV fluorescence colonies coconut agar media (CAM) detect aflatoxin formation. Natural compounds, such as chitosan, have been suggested way prevent fungal infection because their extensive well-known antibacterial action against variety microorganisms. entry morphologically characterize determine aflatoxigenic isolates, nanoparticles using UV–visible, X-ray diffraction (XRD), Energy-dispersive (EDX), scanning electron microscope (SEM) analyses, formulations' vitro antifungal activity treated fungus. Individual concentrations created altering percentages ingredients chitosan solution, nanoparticles, glycerol, canola oil. final solution 20% 2% respectively. Chitosan completely inhibit growth at 2%.